Screening for tetracycline residues in food products of animal origin in the State of Kuwait using Charm II radio-immunoassay and LC/MS/MS methods

Hani M. Al-Mazeedi; Alaa B. Abbas; Hussam F. Alomirah; Wafaa Y. Al-Jouhar; Siham A. Al-Mufty; Mohamed M. Ezzelregal; Rashed A. Al-Owaish

doi:10.1080/19440040903331027

Screening for tetracycline residues in food products of animal origin in the State of Kuwait using Charm II radio-immunoassay and LC/MS/MS methods

Hani M. Al-Mazeedi, Alaa B. Abbas, Hussam F. Alomirah, Wafaa Y. Al-Jouhar, Siham A. Al-Mufty, Mohamed M. Ezzelregal, Rashed A. Al-Owaish

Kuwait Institute for Scientific Research

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

This study surveyed tetracycline residues in foods marketed in Kuwait using various techniques to determine their prevalence above the threshold level. A total of 1517 locally produced and imported food samples of animal origin were collected for analyses, comprising dairy products, eggs and tissue samples (meat, poultry and fish) to give a representative picture of the current use and/or misuse of the drug in Kuwait. Screening was carried out using the Charm II test for tetracycline residues. Positive and suspect positive samples were confirmed by LC/MS/MS. Negative and positive controls, in triplicate, were applied to each method and showed 80-100% agreement. The results showed that 100% of tested eggs, meat, fish, ice cream and cheese were within the limit, while 5% of poultry and 18% of milk samples were above the permitted limit.

Original language	English
Pages (from-to)	291-301
Number of pages	11
Journal	Food Additives and Contaminants
Volume	27
Issue number	3
DOIs	https://doi.org/10.1080/19440040903331027
State	Published - Mar 2010

Keywords

Animal products
Bioassay
Chromatography
Drug residues
Eggs
Fish
Ivermectin
LC/MS
Meat
Oxytetracyclin
Tetracycline
Veterinary drug residues

Funding Agency

Kuwait Foundation for the Advancement of Sciences

Access to Document

10.1080/19440040903331027

Cite this

@article{b85793e387da4b16a7db2d15e0eb62e5,

title = "Screening for tetracycline residues in food products of animal origin in the State of Kuwait using Charm II radio-immunoassay and LC/MS/MS methods",

abstract = "This study surveyed tetracycline residues in foods marketed in Kuwait using various techniques to determine their prevalence above the threshold level. A total of 1517 locally produced and imported food samples of animal origin were collected for analyses, comprising dairy products, eggs and tissue samples (meat, poultry and fish) to give a representative picture of the current use and/or misuse of the drug in Kuwait. Screening was carried out using the Charm II test for tetracycline residues. Positive and suspect positive samples were confirmed by LC/MS/MS. Negative and positive controls, in triplicate, were applied to each method and showed 80-100\% agreement. The results showed that 100\% of tested eggs, meat, fish, ice cream and cheese were within the limit, while 5\% of poultry and 18\% of milk samples were above the permitted limit.",

keywords = "Animal products, Bioassay, Chromatography, Drug residues, Eggs, Fish, Ivermectin, LC/MS, Meat, Oxytetracyclin, Tetracycline, Veterinary drug residues",

author = "Al-Mazeedi, \{Hani M.\} and Abbas, \{Alaa B.\} and Alomirah, \{Hussam F.\} and Al-Jouhar, \{Wafaa Y.\} and Al-Mufty, \{Siham A.\} and Ezzelregal, \{Mohamed M.\} and Al-Owaish, \{Rashed A.\}",

note = "Funding Information: The authors thank the Public Authority for Agriculture and Fisheries Resources (PAAFR), and Public Health Laboratory (PHL), Ministry of Health, Kuwait, for their collaboration with Kuwait Institute for Scientific Research (KISR) in conducting this work. We are thankful to members of the PAAFR research team and PHL for their efforts and contributions throughout this project. The authors are grateful to the Kuwait Foundation for Advancement of Science (KFAS) for partial funding and continued support for the project. The support of the management of the KISR is gratefully acknowledged. Finally, we thank all the project team members for their effort, dedication and hard work.",

year = "2010",

month = mar,

doi = "10.1080/19440040903331027",

language = "English",

volume = "27",

pages = "291--301",

journal = "Food Additives and Contaminants",

issn = "0265-203X",

publisher = "Taylor and Francis Ltd.",

number = "3",

}

TY - JOUR

T1 - Screening for tetracycline residues in food products of animal origin in the State of Kuwait using Charm II radio-immunoassay and LC/MS/MS methods

AU - Al-Mazeedi, Hani M.

AU - Abbas, Alaa B.

AU - Alomirah, Hussam F.

AU - Al-Jouhar, Wafaa Y.

AU - Al-Mufty, Siham A.

AU - Ezzelregal, Mohamed M.

AU - Al-Owaish, Rashed A.

N1 - Funding Information: The authors thank the Public Authority for Agriculture and Fisheries Resources (PAAFR), and Public Health Laboratory (PHL), Ministry of Health, Kuwait, for their collaboration with Kuwait Institute for Scientific Research (KISR) in conducting this work. We are thankful to members of the PAAFR research team and PHL for their efforts and contributions throughout this project. The authors are grateful to the Kuwait Foundation for Advancement of Science (KFAS) for partial funding and continued support for the project. The support of the management of the KISR is gratefully acknowledged. Finally, we thank all the project team members for their effort, dedication and hard work.

PY - 2010/3

Y1 - 2010/3

N2 - This study surveyed tetracycline residues in foods marketed in Kuwait using various techniques to determine their prevalence above the threshold level. A total of 1517 locally produced and imported food samples of animal origin were collected for analyses, comprising dairy products, eggs and tissue samples (meat, poultry and fish) to give a representative picture of the current use and/or misuse of the drug in Kuwait. Screening was carried out using the Charm II test for tetracycline residues. Positive and suspect positive samples were confirmed by LC/MS/MS. Negative and positive controls, in triplicate, were applied to each method and showed 80-100% agreement. The results showed that 100% of tested eggs, meat, fish, ice cream and cheese were within the limit, while 5% of poultry and 18% of milk samples were above the permitted limit.

AB - This study surveyed tetracycline residues in foods marketed in Kuwait using various techniques to determine their prevalence above the threshold level. A total of 1517 locally produced and imported food samples of animal origin were collected for analyses, comprising dairy products, eggs and tissue samples (meat, poultry and fish) to give a representative picture of the current use and/or misuse of the drug in Kuwait. Screening was carried out using the Charm II test for tetracycline residues. Positive and suspect positive samples were confirmed by LC/MS/MS. Negative and positive controls, in triplicate, were applied to each method and showed 80-100% agreement. The results showed that 100% of tested eggs, meat, fish, ice cream and cheese were within the limit, while 5% of poultry and 18% of milk samples were above the permitted limit.

KW - Animal products

KW - Bioassay

KW - Chromatography

KW - Drug residues

KW - Eggs

KW - Fish

KW - Ivermectin

KW - LC/MS

KW - Meat

KW - Oxytetracyclin

KW - Tetracycline

KW - Veterinary drug residues

UR - https://www.scopus.com/pages/publications/76649101454

U2 - 10.1080/19440040903331027

DO - 10.1080/19440040903331027

M3 - Article

C2 - 19894148

AN - SCOPUS:76649101454

SN - 0265-203X

VL - 27

SP - 291

EP - 301

JO - Food Additives and Contaminants

JF - Food Additives and Contaminants

IS - 3

ER -

Screening for tetracycline residues in food products of animal origin in the State of Kuwait using Charm II radio-immunoassay and LC/MS/MS methods

Abstract

Keywords

Funding Agency

Access to Document

Other files and links

Fingerprint

Cite this