Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake

Adnan Husain; Bondi Gevao; Basma Dashti; Abraham Brouwer; Peter Aleaxnder Behnisch; Majed Al-Wadi; Mohamad Al-Foudari

doi:10.1016/j.ecoenv.2013.12.002

Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake

Adnan Husain, Bondi Gevao, Basma Dashti, Abraham Brouwer, Peter Aleaxnder Behnisch, Majed Al-Wadi, Mohamad Al-Foudari

Kuwait Institute for Scientific Research

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data.

Original language	English
Pages (from-to)	27-31
Number of pages	5
Journal	Ecotoxicology and Environmental Safety
Volume	100
Issue number	1
DOIs	https://doi.org/10.1016/j.ecoenv.2013.12.002
State	Published - 2014

Keywords

DR-CALUX bioassay
Feed
Food
PCDD/Fs and dl-PCBs

Funding Agency

Kuwait Foundation for the Advancement of Sciences

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10.1016/j.ecoenv.2013.12.002

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title = "Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake",

abstract = "A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data.",

keywords = "DR-CALUX bioassay, Feed, Food, PCDD/Fs and dl-PCBs",

author = "Adnan Husain and Bondi Gevao and Basma Dashti and Abraham Brouwer and Behnisch, {Peter Aleaxnder} and Majed Al-Wadi and Mohamad Al-Foudari",

year = "2014",

doi = "10.1016/j.ecoenv.2013.12.002",

language = "English",

volume = "100",

pages = "27--31",

journal = "Ecotoxicology and Environmental Safety",

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T1 - Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake

AU - Husain, Adnan

AU - Gevao, Bondi

AU - Dashti, Basma

AU - Brouwer, Abraham

AU - Behnisch, Peter Aleaxnder

AU - Al-Wadi, Majed

AU - Al-Foudari, Mohamad

PY - 2014

Y1 - 2014

N2 - A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data.

AB - A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official maximum limits according to the European Commission (EC) regulations. Suspected and randomly chosen negative samples were analyzed by gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that among suspected samples, one sample was confirmed to be non-compliant. The positive sample was of imported origin. The correlation coefficient of 0.98 between DR-CALUX and GC-HRMS was found. Moreover, the average daily intakes of PCDD/Fs and dl-PCBs for the Kuwaiti population were estimated. Results obtained in this study were discussed and compared with other published data.

KW - DR-CALUX bioassay

KW - Feed

KW - Food

KW - PCDD/Fs and dl-PCBs

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IS - 1

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Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake

Abstract

Keywords

Funding Agency

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