CeO₂-CB nanocomposite as a novel SALDI substrate for enhancing the detection sensitivity of pharmaceutical drug molecules in beverage samples

SALDI-MS analysis of pharmaceutical drug molecules (amitriptyline, imipramine and promazine) using carbon-based substrates, namely, activated charcoal (AC), carbon nanotubes (CNTs), carbon black (CB), graphene (rGO), graphene oxide (GO) and graphite, was explored and compared with the conventional organic matrix of MALDI. CB exhibited superior performance with respect to the other substrates in terms of detection sensitivity. Despite the effectiveness of CB to detect all drug molecules, it demonstrated a number of background signals, which may be an issue for the analysis of other molecules in the future. Therefore, for the first time, a CeO₂-CB nanocomposite was synthesized and applied as a novel SALDI substrate to minimize the background signals and stabilize CB when exposed to high laser power. The nanocomposite was characterized using XRD, TEM, FTIR, UV–Vis and N₂ sorpometry. The spectrum obtained using the novel nanocomposite in the absence of the drug molecules showed minimal background signals compared to CB. Additionally, the CeO₂-CB nanocomposite enhanced the detection sensitivity of the drug molecules with a limit of detection (LOD) of 100 ng/mL. This active substrate nanocomposite was further applied for the analysis of drug-spiked beverages without sample pretreatment or extraction, mimicking cases encountered by forensic toxicologists. All of the drugs and/or their adducts were detected in the drug-spiked beverage samples.