Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait

Salwa Al-Mouqatea; Mohammad Alkhamis; Batool Akbar; Abdulmohsen Ali; Hameed Al-Aqeel; Ahmed Bin-Heji; Mohammed Razzaque; Julio Alvarez; Andres Perez

doi:10.1177/1040638718759574

Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait

Salwa Al-Mouqatea, Mohammad Alkhamis, Batool Akbar, Abdulmohsen Ali, Hameed Al-Aqeel, Ahmed Bin-Heji, Mohammed Razzaque, Julio Alvarez, Andres Perez

Kuwait Institute for Scientific Research

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.

Original language	English
Pages (from-to)	468-470
Number of pages	3
Journal	Journal of Veterinary Diagnostic Investigation
Volume	30
Issue number	3
DOIs	https://doi.org/10.1177/1040638718759574
State	Published - 1 May 2018

Keywords

Bovine tuberculosis
ELISA
gamma interferon
Kuwait
tuberculin

Funding Agency

Kuwait Foundation for the Advancement of Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/1040638718759574

Cite this

Al-Mouqatea, S., Alkhamis, M., Akbar, B., Ali, A., Al-Aqeel, H., Bin-Heji, A., Razzaque, M., Alvarez, J., & Perez, A. (2018). Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait. Journal of Veterinary Diagnostic Investigation, 30(3), 468-470. https://doi.org/10.1177/1040638718759574

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title = "Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait",

abstract = "Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.",

keywords = "Bovine tuberculosis, ELISA, gamma interferon, Kuwait, tuberculin",

author = "Salwa Al-Mouqatea and Mohammad Alkhamis and Batool Akbar and Abdulmohsen Ali and Hameed Al-Aqeel and Ahmed Bin-Heji and Mohammed Razzaque and Julio Alvarez and Andres Perez",

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Al-Mouqatea, S, Alkhamis, M, Akbar, B, Ali, A, Al-Aqeel, H, Bin-Heji, A, Razzaque, M, Alvarez, J & Perez, A 2018, 'Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait', Journal of Veterinary Diagnostic Investigation, vol. 30, no. 3, pp. 468-470. https://doi.org/10.1177/1040638718759574

Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait. / Al-Mouqatea, Salwa; Alkhamis, Mohammad; Akbar, Batool et al.
In: Journal of Veterinary Diagnostic Investigation, Vol. 30, No. 3, 01.05.2018, p. 468-470.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait

AU - Al-Mouqatea, Salwa

AU - Alkhamis, Mohammad

AU - Akbar, Batool

AU - Ali, Abdulmohsen

AU - Al-Aqeel, Hameed

AU - Bin-Heji, Ahmed

AU - Razzaque, Mohammed

AU - Alvarez, Julio

AU - Perez, Andres

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.

AB - Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.

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KW - ELISA

KW - gamma interferon

KW - Kuwait

KW - tuberculin

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AN - SCOPUS:85046846487

SN - 1040-6387

VL - 30

SP - 468

EP - 470

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

IS - 3

ER -

Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait

Abstract

Keywords

Funding Agency

UN SDGs

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