A hemolytic protein secreted from epidermal cells of the arabian gulf catfish, Arius Thalassinus (Ruppell)

1. 1. A fright or shock induced toxic secretion (gel) from the epidermis of the Arabian Gulf catfish, Arius thalassinus, exhibits hemolytic activity when tested against red blood cells from many different sources. 2. 2. An enzyme with hemolytic activity, which represents 1.1% of the total soluble gel protein fraction, has been purified to homogeneity. 3. 3. Molecular sieve chromatography and SDS polyacrylamide gel electrophoresis of the purified protein indicate a mol. wt of 34,000. 4. 4. One additional protein component with hemolytic activity was found in the epidermal secretion. 5. 5. Specific activity of the catfish epidermal factor is 20.6 units/mg protein, a level somewhat lower than those of most protein hemolytic factors. 6. 6. The catfish hemolytic factor was not ichthyotoxic when tested against small fish and did not cause lethality when administered intravenously to rabbits.