Project Details
Abstract English
Physiological Adaptation of Cardiac and Skeletal Muscle to Hypergravity in Aerobic and Anaerobic Exercise in Small Animals
The aim of this study is to investigate the effects of hypergravitation (all day overloading) and high intensity Berobic and anaerobic running trainings on skeletal muscle metabolism, muscle fther characteristics, muscle mass, threshold level and conduction velocity for rats, normal and cardiac myopathic hamsters.
The cardiac muscle hypertrophy and compliance will also be determined. Hypergravitation is attained by fastening extra loads (15% of the body weight) to the animals which will wear them for eight weeks. Running speed for training will be determined on the basis of individual maximum running speed (Vmax). In anaerobic training 90-95% of Vmax will be used and 30-60% of Vmax for the animals involved in aerobic training. Work Toads will be increased weekly depending on the actual condition of the animals. Duration of the training period is eight weeks. The animals will be divided into six groups (one control and five experimental) each consisting eight animals, respectively. Normal Syrian and BIO 14.6 cardiomyopathic hamsters as well as Wistar rats will be used.
The animals will be tested before and after the experiment for estimation of Vmax, aerobic and anaerobic capacity. After experiment the animals will be anesthetized while threshold level and conduction velocity is determined for gastrocnemius and soleus muscles. Gastrocnemius and soleus muscles from both legs will be removed for nistochemical, biochemical and morphological studies. Muscle fiber composition, mean cross-sectional area for both fast and slow fibers, Ca2+ activated myofibrillar ATP-ase activity, Tactic dehydrogenase, phosphofructokinse, citrate synthestase, succinic dehydrogenase and creatin kinase enzyme activities will be determind in both gastrocnemius and solues muscles. Muscle mass, and cross-sectional area will also be determined. Cardiac output, left intraventr
The aim of this study is to investigate the effects of hypergravitation (all day overloading) and high intensity Berobic and anaerobic running trainings on skeletal muscle metabolism, muscle fther characteristics, muscle mass, threshold level and conduction velocity for rats, normal and cardiac myopathic hamsters.
The cardiac muscle hypertrophy and compliance will also be determined. Hypergravitation is attained by fastening extra loads (15% of the body weight) to the animals which will wear them for eight weeks. Running speed for training will be determined on the basis of individual maximum running speed (Vmax). In anaerobic training 90-95% of Vmax will be used and 30-60% of Vmax for the animals involved in aerobic training. Work Toads will be increased weekly depending on the actual condition of the animals. Duration of the training period is eight weeks. The animals will be divided into six groups (one control and five experimental) each consisting eight animals, respectively. Normal Syrian and BIO 14.6 cardiomyopathic hamsters as well as Wistar rats will be used.
The animals will be tested before and after the experiment for estimation of Vmax, aerobic and anaerobic capacity. After experiment the animals will be anesthetized while threshold level and conduction velocity is determined for gastrocnemius and soleus muscles. Gastrocnemius and soleus muscles from both legs will be removed for nistochemical, biochemical and morphological studies. Muscle fiber composition, mean cross-sectional area for both fast and slow fibers, Ca2+ activated myofibrillar ATP-ase activity, Tactic dehydrogenase, phosphofructokinse, citrate synthestase, succinic dehydrogenase and creatin kinase enzyme activities will be determind in both gastrocnemius and solues muscles. Muscle mass, and cross-sectional area will also be determined. Cardiac output, left intraventr
| Status | Finished |
|---|---|
| Effective start/end date | 1/11/87 → 1/11/89 |
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