ABI Fast Real Time PCR - Applied Bio Systems ViiA

Features: • Optics: 6 emission filter (500-720 nm), 6 excitation filters (450-670 nm), OptiFlextm System (enhanced flourescence detection). • Temperature Range & Uniformity: 4-100°C, ±0.5°C, within 30 sec of reaching temprature set point. • Data Collection: Multiple ramp method detection formats provide more flexibilty for collecting dat during a ramp stage. • Dynamic Range: Up to 9 logs of linear dynamic range. • Detection Method: Primer - Probe Detection, SYBR. • Precise Quantification: Detect changes as small as 1.5-fold in target quantities in singleplex reactions. • Run Time: About 35 min. (Varies depending on reagents and other factors). Applications: • Absolute quantitation (standard curve). • Relative quatitation (ΔΔCt or Comparativve Ct). • SYBR green assay. • Allelic discrimination (SNP Genotyping). • Plus Minus Assay (Presence/ Absence). • Qualitative Analysis. • High Resolution Melting (HRM) analysis.